Photosynthesis and Respiration Experiment Questions
Assignment ID Number AFFGEHU83939HD Type of Document Essay Writing Format APA/MLA/Harvard Academic Level Masters/University References/Sources 4 References
Photosynthesis and Respiration Experiment Questions
questions to answer:
- What does CO2indicate in these tubes/cups? How did you measure the amount of CO2 produced?
- In which set up did you observe the maximum amount of respiration? Explain your answer.
- If we boiled the yeast, sugar and water mixture for 20 minutes and performed the same experiment, how much of CO2do you expect to be produced. Give explanation for your answer
- Is light necessary in photosynthesis? __________________________________________
Briefly describe the two reactions of photosynthesis and explain their dependence on light:
- Explain your conclusion about the occurrence of photosynthesis in setup 1.
- Explain your conclusion about the occurrence of photosynthesis in setup 2.
- What would happen to the life on earth if all processes of photosynthesis stop?
- Use colored pencils to draw your chromatography paper results and label the pigments. Upload a picture of your drawing here.
- The main pigments colors you observed in the leaves are ______________________________
What are their names? ______________________________________________________
Experimentation Online- Photosynthesis and Respiration
PART I- CELLULAR RESPIRATION
Yeast are single-celled fungi. The species called Saccharomyces cerevisiae is commonly called Baker’s or Brewer’s yeast. Like other eukaryotes with mitochondria, yeast can use oxygen to generate ATP in the process of oxidative phosphorylation. These yeast are facultative aerobes which means they can also switch to an anaerobic mechanism of ATP production called fermentation. In all organisms, the process of glycolysis occurs anaerobically in the cytoplasm to produce two pyruvate molecules from a single glucose. This process produces 2 new ATP molecules and reduced nicotinamide adenine dinucleotide (NADH).
Fermentation is an anaerobic process that occurs in the cytoplasm and quickly generates an additional ATP through the reduction of pyruvate. NADH is the source of electrons in this process that is oxidized to NAD+. Many organisms will ferment to generate lactic acid and CO2 from the pyruvate in order to generate ATP. Yeast fermentation produces ethanol and CO2. as byproducts.
In this experiment, we will use the amount of CO2 produced by a sample of yeast to measure the amount of fermentation. CO2 produced could be seen as bubbles in the reaction mixture.
3 tablespoons active dry yeast
3 teaspoons sugar
1/4 cup water
warm water in a big pan
Ice-cold water in a big pan
Transparent Disposable Cup
- Mix 1 tablespoon yeast and 1 teaspoon sugar with 1/4 cup water in three separate transparent disposable cups.
- Keep the first cup at room temperature, the second one in the pan containing ice-cold water and the third cup in a pan containing warm water.
- Record the amount of bubbles in all three cups at the start of the experiment or time zero.
- Record the amount of bubbles in all three cups after 30 minutes
- Answer questions in the Post-Lab Report.
PART II- PHOTOSYNTHESIS
Fresh or frozen spinach leaves
Alcohol (70% rubbing alcohol)
Acetone nail polish remover
Small glass jars with lid (sauce jar or baby food jar)
Lamp (blacklight /UV flashlight)
Coffee filter paper
- Observing photosynthesis and the necessity of light in the process
Enter this virtual lab (Links to an external site.) to study photosynthesis.
Lab setup 1: in the setting area, set “stop-at” at 2000, “Initial # of chloroplasts” at 3, “initial carbon dioxide” at 100, “water flow through leaf” at 25, “intensity of light” at 50, and check “auto-stop”.
Virtual lab setup 1 Virtual lab setup 2
Click on the “Setup/reset” button and record the initial numbers of the “carbon dioxide molecules”, “oxygen molecules”, and “sugar molecules produced” in section A of the lab report.
Click “go/pause” button to start the simulation. Observe the consumption of carbon dioxide and production of oxygen and sugar molecules in chloroplasts (green circles) during photosynthesis.
After the simulation stops, record the number molecules in the lab report (final number).
Now use lab setup2 to study the necessity of light. You can remove the light by directly typing “0” in the box of “intensity of light” or move the slider all the way to the left until it reads at 0.
Click on the “Setup/reset” button and record the initial numbers of the “carbon dioxide molecules”, “oxygen molecules”, and “sugar molecules produced” of setup 2 in the lab report.
Click “go/pause” button to start the simulation.
After the simulation stops, record the number molecules (final number of setup 2).
Complete related questions in the lab report.
- Extract chlorophyll and observe its fluorescence
When a leaf is exposed to light, its chlorophyll absorbs the light energy. This energy pushes the electron of chlorophyll into an excited state. The excited electron then transfers the energy from one molecule to another molecule in the thylakoid membrane to process photosynthesis. While in a situation where the light energy absorbed by chlorophyll cannot be pass on to perform photosynthesis, the electron will return to its original energy state, and in the process, release the light energy, and thus fluoresces. We will observe the fluorescence of chlorophyll in this experiment.
Use a scissor to cut five spinach leaves into very small pieces.
Place the leave pieces in a glass jar.
Add about 2 tablespoon of rubbing alcohol, soak the leaves pieces for 2 days or till the liquid is dark green.
Note: Alcohol is flammable – use it with care. As an organic solvent, alcohol dissociates the thylakoid membrane and dissolve the chlorophylls from the membrane.
Pour the liquid through a coffee filter paper and collect the extract to a small glass jar.
Turn off the room lights, shine a blacklight on to the extract and observe the color it fluoresce.
Complete section B of the lab report.
If you don’t have the materials, watch thevideo (Links to an external site.)here and complete section B of the lab report.
- Separate plant pigments by paper chromatography
Cut a strip of coffee filter paper long enough to stand in a glass jar as shown above.
Cut a point at the end of the paper.
Place a spinach leaf on the filter paper at the pointed end. Press and roll the edge of a coin over the leaf to crush the cells and form a stripe of pigment about 2 cm from the pointed end of the paper. Blow the stripe dry completely, and repeat this step at least 10 times at the same location on the filter paper until one dark concentrated stripe is created.
Note: use medium force to press the coin on the leaves to avoid breaking through the paper.
Add acetone nail polish remover to the glass jar for about 1 cm deep.
Note: Acetone is flammable – use it with care.
Slowly and carefully insert the filter paper with the pointed end towards the bottom in the jar with acetone solvent. Make sure that the dark stripe of plant extract is above the solvent.
Cap the jar and observe the solvent move up the paper until it comes within 1 cm from the top.
Remove the paper and immediately use a pencil to mark the position of the solvent.
Place the coffee filter paper on a paper towel to let it dry.
Observe your filter paper and locate the orange carotene at the top, followed by the yellow xanthophyll, the blue-green chlorophyll a, and the yellow-green chlorophyll b.
Draw a colored picture of your observation and upload the image to question No. 9 in the lab report. Also answer other relevant questions in the lab report.
BIO 130 Basic Chemistry for Biology Worksheet
BASIC CHEMISTRY FOR BIOLOGY WORKSHEET
Each of the following elements is found in living organisms. For each symbol shown, name the element, list the atomic number, the atomic mass, the number of protons, neutrons and electrons.
Symbol Element Atomic # Mass # Protons # Neutrons # Electrons
Atoms normally contain equal numbers of protons and electrons, thus they are electrically ( positive / negative / neutral ) .
An electron is a (negatively / positively) charged particle. So if an atom loses an electron, it will then have an overall (negative / positive ) charge.
If an atom were to gain an electron, it would have an overall (negative / positive ) charge.
If a hydrogen atom loses its electron, it ends up with a (negative / positive ) charge. (This hydrogen ion is often referred to as a proton, as the entire structure of the atom is now simply the proton that is left in its nucleus)
The atomic symbol for magnesium is ___ _________.
How many protons does a magnesium atom have? _____ How many neutrons? _____
How many electrons? ____________
In the space below, draw a magnesium atom. Label the protons, neutrons, and place the electrons in orbitals with the correct number of electrons in each. Remember, the first orbital can take 2 electrons, the second can take 8 and the third can take 8.
Atoms with 1,2 or 3 electrons in their valence shell (the outermost shell) will lose electrons in order to become stable, thus forming positively charged ions. What will happen to the magnesium atom?
In the space below, draw a chlorine atom with the protons, neutrons and electrons correctly placed in orbitals.
Atoms with 6 or 7 electrons in their valence shell will gain electrons in order to become stable, thus forming negatively charged ions. What will happen to the chlorine atom?
If magnesium and chlorine atoms were to come into proximity with each other, what would be the formula of the molecule that would be formed?
For each of the following molecules, list the elements that are present, the number of atoms of each and draw the structure of the molecule.
Molecule Elements Present & of Atoms Structure_______
List the elements contained in each of the following molecules:
Biotin (one of the B-vitamins) C10H16O3N2S
Adenosine Triphosphate (the body’s primary energy carrying compound) C11H18O13N5P3
Chlorophyll (the primary pigment in photosynthesis) C55H68O5N4Mg
Write the formula for the following molecules:
A molecule of sucrose with 12 Carbon atoms, 22 Hydrogen atoms, and 11 Oxygen atoms
A molecule of vitamin B12 with 63 Carbon atoms, 90 Hydrogen atoms, 14 Oxygen atoms, 14 nitrogen atoms, 1 phosphorous atom, and 1 atom of Cobalt
What do each of the following formulas represent?
6 H2O __________________ 4O2 _______________ 3NH3____________
QUALITY OF RESPONSE NO RESPONSE POOR / UNSATISFACTORY SATISFACTORY GOOD EXCELLENT Content (worth a maximum of 50% of the total points) Zero points: Student failed to submit the final paper. 20 points out of 50: The essay illustrates poor understanding of the relevant material by failing to address or incorrectly addressing the relevant content; failing to identify or inaccurately explaining/defining key concepts/ideas; ignoring or incorrectly explaining key points/claims and the reasoning behind them; and/or incorrectly or inappropriately using terminology; and elements of the response are lacking. 30 points out of 50: The essay illustrates a rudimentary understanding of the relevant material by mentioning but not full explaining the relevant content; identifying some of the key concepts/ideas though failing to fully or accurately explain many of them; using terminology, though sometimes inaccurately or inappropriately; and/or incorporating some key claims/points but failing to explain the reasoning behind them or doing so inaccurately. Elements of the required response may also be lacking. 40 points out of 50: The essay illustrates solid understanding of the relevant material by correctly addressing most of the relevant content; identifying and explaining most of the key concepts/ideas; using correct terminology; explaining the reasoning behind most of the key points/claims; and/or where necessary or useful, substantiating some points with accurate examples. The answer is complete. 50 points: The essay illustrates exemplary understanding of the relevant material by thoroughly and correctly addressing the relevant content; identifying and explaining all of the key concepts/ideas; using correct terminology explaining the reasoning behind key points/claims and substantiating, as necessary/useful, points with several accurate and illuminating examples. No aspects of the required answer are missing. Use of Sources (worth a maximum of 20% of the total points). Zero points: Student failed to include citations and/or references. Or the student failed to submit a final paper. 5 out 20 points: Sources are seldom cited to support statements and/or format of citations are not recognizable as APA 6th Edition format. There are major errors in the formation of the references and citations. And/or there is a major reliance on highly questionable. The Student fails to provide an adequate synthesis of research collected for the paper. 10 out 20 points: References to scholarly sources are occasionally given; many statements seem unsubstantiated. Frequent errors in APA 6th Edition format, leaving the reader confused about the source of the information. There are significant errors of the formation in the references and citations. And/or there is a significant use of highly questionable sources. 15 out 20 points: Credible Scholarly sources are used effectively support claims and are, for the most part, clear and fairly represented. APA 6th Edition is used with only a few minor errors. There are minor errors in reference and/or citations. And/or there is some use of questionable sources. 20 points: Credible scholarly sources are used to give compelling evidence to support claims and are clearly and fairly represented. APA 6th Edition format is used accurately and consistently. The student uses above the maximum required references in the development of the assignment. Grammar (worth maximum of 20% of total points) Zero points: Student failed to submit the final paper. 5 points out of 20: The paper does not communicate ideas/points clearly due to inappropriate use of terminology and vague language; thoughts and sentences are disjointed or incomprehensible; organization lacking; and/or numerous grammatical, spelling/punctuation errors 10 points out 20: The paper is often unclear and difficult to follow due to some inappropriate terminology and/or vague language; ideas may be fragmented, wandering and/or repetitive; poor organization; and/or some grammatical, spelling, punctuation errors 15 points out of 20: The paper is mostly clear as a result of appropriate use of terminology and minimal vagueness; no tangents and no repetition; fairly good organization; almost perfect grammar, spelling, punctuation, and word usage. 20 points: The paper is clear, concise, and a pleasure to read as a result of appropriate and precise use of terminology; total coherence of thoughts and presentation and logical organization; and the essay is error free. Structure of the Paper (worth 10% of total points) Zero points: Student failed to submit the final paper. 3 points out of 10: Student needs to develop better formatting skills. The paper omits significant structural elements required for and APA 6th edition paper. Formatting of the paper has major flaws. The paper does not conform to APA 6th edition requirements whatsoever. 5 points out of 10: Appearance of final paper demonstrates the student’s limited ability to format the paper. There are significant errors in formatting and/or the total omission of major components of an APA 6th edition paper. They can include the omission of the cover page, abstract, and page numbers. Additionally the page has major formatting issues with spacing or paragraph formation. Font size might not conform to size requirements. The student also significantly writes too large or too short of and paper 7 points out of 10: Research paper presents an above-average use of formatting skills. The paper has slight errors within the paper. This can include small errors or omissions with the cover page, abstract, page number, and headers. There could be also slight formatting issues with the document spacing or the font Additionally the paper might slightly exceed or undershoot the specific number of required written pages for the assignment. 10 points: Student provides a high-caliber, formatted paper. This includes an APA 6th edition cover page, abstract, page number, headers and is double spaced in 12’ Times Roman Font. Additionally, the paper conforms to the specific number of required written pages and neither goes over or under the specified length of the paper.
GET THIS PROJECT NOW BY CLICKING ON THIS LINK TO PLACE THE ORDER
Do You Have Any Other Essay/Assignment/Class Project/Homework Related to this? Click Here Now [CLICK ME]and Have It Done by Our PhD Qualified Writers!!
Photosynthesis and Respiration Experiment Questions
Tired of getting an average grade in all your school assignments, projects, essays, and homework? Try us today for all your academic schoolwork needs. We are among the most trusted and recognized professional writing services in the market.
We provide unique, original and plagiarism-free high quality academic, homework, assignments and essay submissions for all our clients. At our company, we capitalize on producing A+ Grades for all our clients and also ensure that you have smooth academic progress in all your school term and semesters.
High-quality academic submissions, A 100% plagiarism-free submission, Meet even the most urgent deadlines, Provide our services to you at the most competitive rates in the market, Give you free revisions until you meet your desired grades and Provide you with 24/7 customer support service via calls or live chats.